Plant & Animal Genome
Genotyping By Sequence
Genotyping by sequencing (GBS) is a rapid and cost-effective approach for comparing genotypes through the mapping of large numbers of SNPs or other markers. A restriction enzyme digestion step reduces genome complexity, so GBS can be applied to large genomes, and the end reads of the restriction fragments allow variants to be compared when no reference genome is available.
Applications of genotyping by sequencing include tracking plant and animal genotypes in breeding programs and conservation projects, examining the diversity of natural populations, discovery of new genetic markers, and screening variants prior to whole genome resequencing.
NovogeneAIT provides both GBS and WGS services, and can advise you on the best method for your project and budget goals. GBS provides less sequence data than WGS, but is more cost-effective for some applications, is highly accurate, and can be used in the absence of a reference genome. When a reference genome is available, WGS is recommended for more comprehensive comparisons.
Whole Genome Resequencing
With advancements in next-generation sequencing technology, whole genome resequencing (WGS) has become the most rapid and effective method to unravel, at the genomic level, the underlying mechanisms of species origin, development, growth, and evolution. Using WGS, the complete genome data from one or more variants can be aligned to known genomic sequence(s) for the species. Applications of WGS include detection of genetic differences between variants, transposon fingerprinting for assessing germplasm diversity and lineages, and mapping loci associated with specific traits, such as disease resistance.
NovogeneAIT is highly experienced in the applications of WGS for characterizing plant and animal variants. With cutting-edge Illumina HiSeq X™ Five platform and our expert bioinformatics analysis, we provide researchers with high quality data in a highly cost-effective manner. Bioinformatics analysis includes but not limited to detecting SNPs, InDels, structure variations, and copy number variations with high accuracy and verification rates.
The pan-genome is the superset of all the genes present within a species, ranging from the “core genome” genes shared by all strains to “dispensable genome” or “unique genes” present in subset of strains or only in an individual strain.
Next-generation sequencing and state-of-the-art assembly algorithms make the construction of pan-genome maps feasible, and the construction of genome maps for individual or multiple strains provides unprecedented opportunities to investigate detailed genetic diversity at the population level. Examples of applications include breeding studies for identifying the genetic basis for desirable traits in wild and cultivated strains, and molecular ecology studies on the adaptive evolution of species across different habitats.
NovogeneAIT is a pioneer and world leader in the assembly and analysis of pan-genome maps. Our pan-genomic research studies have been published in leading scientific journals, and we provide our customers with the same high-quality data and analyses.
De Novo Sequencing
With de novo sequencing, the first genome map for a species is generated, providing a valuable reference sequence for phylogenetic studies, analysis of species diversity, mapping of specific traits and genetic markers, and other genomics research.
With the development of next-generation sequencing technology, de novo sequencing has become more rapid and affordable, and Novogene is at the forefront of this technology. Novogene’s founder, Dr. Ruiqiang Li, is a leading genomics expert and a primary developer of the SOAPdenovo software package for genome assembly. Dr. Li and the Novogene team have contributed to several important publications on novel genome sequences, and we can provide you with the high level of expertise required for your specific project.
NovogeneAIT offers de novo sequencing services using various platforms including Illumina, PacBio and 10X Genomics. For each project, our scientists will design the best sequencing strategy utilizing an optimal combination of short reads and long range sequence information to achieve the most comprehensive de novo assembly results for your genome of interest.