Gene Regulation

Gene Regulation

mRNA-Seq

mRNA sequencing (mRNA-Seq), a method of transcriptome profiling based on deep-sequencing technologies, provides a more precise measurement of transcript levels and their isoforms with a complete snapshot of the coding transcriptome than other methods such as microarray analysis. Transcriptome sequencing with longer read length enables the identification of novel transcripts, alternative splicing and gene fusions, and scientists can identify biomarkers or key regulated genes, key functional genes related to different phenotypes, as well as key factors in temporal changes. RNA-Seq quantification with shorter read length can simultaneously measure the expression levels of many transcripts. It is widely used in disease research, drug response research, pharmacokinetics, and personalized healthcare reasearch.

NovogeneAIT offers comprehensive transcriptome sequencing and RNA-Seq quantification services using cutting edge Illumina platform and widley accepted analysis software, serving customers with different research emphases.

LncRNA-Seq

Long non-coding RNAs (LncRNAs) are transcribed RNAs longer than 200 nt that do not encode for proteins. LncRNAs are involved in a wide range of important cellular processes such as X-chromosome inactivation, imprinting, maintenance of pluripotency, lineage commitment and apoptosis. LncRNAs are also known to be involved with human diseases such as cancer, cardiovascular disease and neurological disorders, and are of significant interest to researchers.

NovogeneAIT bioinformatics team composed entirely of PhDs provides comprehensive analysis for both lncRNAs and mRNAs, enabling access to lncRNA and mRNA information in a single sequencing run. Applications include comparison of lncRNA and mRNA expressions in different stages of development and in different tissues, as well as unveiling key functions of mRNAs and lncRNAs.

Small RNA Sequencing

Small RNAs are a type of non-coding RNA of 50-250 nucleotides in length that regulate the translation of target mRNAs. Small RNA sequencing, including the sequencing of microRNA (miRNA-Seq), is a valuable method for investigating the function of small RNAs and for constructing regulatory networks of miRNAs and their target genes.

NovogeneAIT offers comprehensive small RNA sequencing and bioinformatics analysis for miRNA and other types of small RNAs, including siRNA and piRNA. Scientists at NovogeneAIT use the cutting-edge Illumina platform, with the SE50 sequencing strategy and widely accepted analysis software, to support RNA research on a wide range of biological systems. Please contact us for more information.

Whole Genome Bisulfate Sequencing

DNA methylation at the C5 position of cytosine plays a crucial role in gene expression and chromatin remodelling, and perturbations in methylation patterns are implicated in the development of cancer, neurodegenerative diseases, and neurological disorders. Therefore, the mapping of methylated bases (the methylome) is critical to understanding gene expression and other processes subject to epigenetic regulation.

NovogeneAIT provides whole genome sequencing of bisulfite-converted DNA, as an effective method to identify individually methylated cytosines on a genome-wide scale. Methylome analysis is an increasingly valuable research tool with a range of applications, including studies on gene regulation, stem cell differentiation, embryogenesis, aging, cancer and other diseases, and phenotypic diversity and evolution in plants and animals.

ChIP-Seq

ChIP-Seq provides genome-wide profiling of DNA targets for histone modification, transcription factors, and other DNA-associated proteins; it combines the selectivity of chromatin immunoprecipitation (ChIP) for recovering specific protein-DNA complexes with the power of NGS for high-throughput sequencing of the recovered DNA. Additionally, because the protein-DNA complexes are recovered from living cells, binding sites can be compared in different cell types or tissues, or under different conditions.

In ChIP-Seq, enriched DNA regions (protein binding sites) are detected as peaks above background reads, and bioinformatics analyses of these regions can reveal binding motifs. Applications include studies on gene regulation, transcription complex assembly, histone modification, developmental mechanisms, and disease processes. At Novogene, we can provide you with high quality sequencing and comprehensive bioinformatics analysis for your ChIP-Seq project.

NovogeneAIT also provides sequencing service and bioinformatics analysis on other kinds of IP-sequencing. Please contact us for more information.


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